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    Zymo Research solution zymo research cat
    Solution Zymo Research Cat, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 3380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solution+zymo+research+cat/DNA%2FRNA+Shield/pm41004341-668-163-164
    Average 99 stars, based on 3380 article reviews
    solution zymo research cat - by Bioz Stars, 2026-10
    99/100 stars

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    Saline:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.

    Quantitative RT-PCR:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.

    Rapid Amplification of cDNA Ends:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.

    Plasmid Preparation:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.

    Microarray:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.

    Sequencing:

    Article Title: Quorum-sensing control of sepsis in the coagulase-negative staphylococcal species Staphylococcus haemolyticus.
    Article Snippet: Δpsmβ pTX Δ psmβ3 This study N/A Staphylococcus haemolyticus isolate 1272 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1614 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 1644 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2152 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2352 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 2475 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3486 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3690 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3815 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus haemolyticus isolate 3836 Gift from Dr. H. Rohde. (University of Hamburg, Hamburg, Germany) N/A Staphylococcus aureus RN4220 De Azavedo et al. 74 N/A S. carnosus TM300 Gotz 75 N/A E. coli DH5α Invitrogen CAT#18265017 Biological samples Human blood This paper N/A Chemicals, peptides, and recombinant proteins RPMI 1640 without phenol red Gibco CAT#11835-030 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Gibco CAT#15630-080 Tryptone Soy Broth Difco CAT#211823 Agar BD CAT#281230 (Continued on next page) Cell Reports 44, 116349, October 28, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline (PBS) no Mg or Ca Gibco CAT#10010-023 Dulbecco’s PBS Gibco CAT#14190-144 Dextran Sigma-Aldrich CAT#D8802 Ficoll-Paque PLUS Cytiva CAT#17144002 Triton X-100 Sigma-Aldrich CAT#T8787-100ML Tetracycline Sigma-Aldrich CAT#T3383 Ampicillin sodium salt Sigma-Aldrich CAT#A9518 Chloramphenicol Sigma-Aldrich CAT#C0378 Anhydrotetracycline Sigma-Aldrich CAT#37919 Yeast extract Sigma-Aldrich CAT#41625 Tryptone BD CAT#211705 Sodium Chloride Sigma-Aldrich CAT#3624-01 Non-fat powdered milk Saco Foods N/A Fluo3-AM Invitrogen CAT#F1242 Gentamicin Gibco CAT#15710-064 Saline ICU Medical CAT#798309 Acetonitrile Honeywell CAT#349984X4L N-Formylated PSMα peptide Peptide 2.0 N/A N-Formylated PSMβ1 peptide Peptide 2.0 N/A N-Formylated PSMβ2 peptide Peptide 2.0 N/A N-Formylated PSMβ3 peptide Peptide 2.0 N/A Trifluoroacetic acid Sigma-Aldrich CAT#A116-50 Critical commercial assays Human IL-8/CXCL8 DuoSet ELISA kit R&D systems CAT#DY208 Mouse CXCL-1 DuoSet ELISA kit R&D systems CAT#DY453 Mouse TNF⍺ DuoSet ELISA kit R&D systems CAT#DY410 Ovation® Pico WTA System V2 Nugen CAT#3302 Gateway BP clonase Invitrogen CAT#11789-013 Cytotoxicity Detection Kit (LDH) Roche CAT#11644793001 SuperScript TM III Platinum TM SYBR TM Green One-Step qRT-PCR Kit Invitrogen CAT#11736-059 DNA/RNA Shield Ready-to-use solution Zymo Research CATR1100-50 5ʹ RACE System For Rapid Amplification of cDNA Ends kit Invitrogen CAT#18374058 T4 RNA ligase 1 NEB CAT#M0204S Calf-intestinal alkaline phosphatase NEB CAT#M0525S DNAse Kit Amersham CAT#27051401 RNeasy Mini Kit Qiagen CAT#74106 Plasmid Midi Kit Qiagen CAT#12145 Deposited data Raw and analyzed microarray data This paper GEO: GSE285447 Whole genome sequencing data This paper BIOPROJECT: PRJNA1255573 Experimental models: Organisms/strains Mouse C57BL-6Ncrl Charles River Laboratories Strain code 027, RRID: IMSR_CRL:027 Oligonucleotides See Table S1 (Continued on next page) 14 Cell Reports 44, 116349, October 28, 2025 .. A human S. haemolyticus skin isolate (type strain ATCC 29970) 73 was used as the background for the generation of isogenic mutants.



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